mab93081  (R&D Systems)

Journal: Frontiers in Psychiatry

Article Title: Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder

doi: 10.3389/fpsyt.2022.577857

Figure Lengend Snippet: Western blot validations of potential biomarkers for paroxetine treatment response in MDD patients.

Article Snippet: After blocking in 2% BSA in PBST for 1 h at room temperature, the PVDF membrane was incubated for 10 h at 4°C with the following primary antibodies: anti-hydroxypyruvate isomerase (HYI) antibody (Abcam, Cambridge, UK), anti-alpha cardiac muscle 1 actin (ACTC1) antibody (R&D Systems, Minneapolis, MN, USA), anti-eukaryotic translation initiation factor 4H (eIF4H) antibody (Thermo Fisher Scientific, Waltham, MA, USA), anti-adenylate kinase isoenzyme 1 (AK1) antibody (Thermo Fisher Scientific, Waltham, MA, USA), anti-mitochondrial dynamin-like 120 kDa protein (OPA1) antibody (R&D Systems, Minneapolis, MN, USA), and anti-RNA binding motif 8A (RBM8A) antibody (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Western Blot

Journal: Frontiers in Psychiatry

Article Title: Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder

doi: 10.3389/fpsyt.2022.577857

Figure Lengend Snippet: Western blots of 10 MDD patients with paroxetine reponse, before and after antidepressant treatment. A, after treatment; ACTC1, alpha cardiac muscle 1 actin; AK1, adenylate kinase isoenzyme 1; B, before treatment; COX-IV, cytochrome c oxidase subunit IV, as control; EIF4H, eukaryotic translation initiation factor 4H; HYI, putative hydroxypyruvate isomerase; MDD, major depressive disorder; OPA1, mitochondrial dynamin-like 120 kDa protein; RBM8A, RNA binding motif 8A.

Article Snippet: After blocking in 2% BSA in PBST for 1 h at room temperature, the PVDF membrane was incubated for 10 h at 4°C with the following primary antibodies: anti-hydroxypyruvate isomerase (HYI) antibody (Abcam, Cambridge, UK), anti-alpha cardiac muscle 1 actin (ACTC1) antibody (R&D Systems, Minneapolis, MN, USA), anti-eukaryotic translation initiation factor 4H (eIF4H) antibody (Thermo Fisher Scientific, Waltham, MA, USA), anti-adenylate kinase isoenzyme 1 (AK1) antibody (Thermo Fisher Scientific, Waltham, MA, USA), anti-mitochondrial dynamin-like 120 kDa protein (OPA1) antibody (R&D Systems, Minneapolis, MN, USA), and anti-RNA binding motif 8A (RBM8A) antibody (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Western Blot, Control, RNA Binding Assay